This'd be a dream for me atm...
Mine rolled into the tuner with 1/4 tank of ~P100, drained with the fuel pump and topped off with E85... so the tune was done in warm weather with ~10% Petrol
Started up 2nd or 3rd rotation of the engine on the first crank in the following weeks (barely driven), but after the next track day (and multiple tanks of E85 bringing it back to a Pure mixture) it turned to crap.
4-5 Cranks of ~5 seconds each... not even giving a cough until the 3rd crank, and once it was running I could even touch the throttle until the water temp got up to 40/50c, anything more than 10% throttle would cause the engine to choke out and revs to drop to stalling danger zone.
Once running and warm, it was perfect for the rest of the day... But trying to cold start an E85 car with an incomplete cold start tune, before 6am, with a Ti straight through exhaust... It's not my idea of a fun time. I'm sure it'll be perfect after dropping it back into the tuner for a few days to get a touch up now that the variables (fuel and temp) are correct...
But Birds is right, E85 doesn't have the appeal it used to for street cars, mainly due to the price coming back up so the savings no longer counter the inconvenience and increased usage (reducing range)